Understanding Thawing Protocols

What is a thawing protocol?

We use the term 'thawing protocol' to describe a combination of device settings which determine how cryopreserved samples will be thawed, and also what thresholds might trigger an alarm. At the time of writing, a thaw protocol includes:

• A name
• Overwrap or no overwrap
• Fill Volume
• Load Alarm temperature
• Time Alarm duration

How many thawing protocols can be stored on a thawing system?

8 protocols can be stored.

Why are the thawing protocols on my device different to the user guide?

Thawing protocols shown in the user guide are examples of what can be created by appropriately authorised users. The default set available on a new system may vary depending on when it was manufactured and what test programs it ran during our quality control procedures. Typically customers will want to replace these default protocols with those they have validated for their own cell lines and processes. This can be done manually on the device, or more conveniently via a centralised web application: my.Cryochain. Please see the next question for more details.

How can I edit thawing protocols?

The easiest way to do this is by synchronising the Thawing System to the my.Crychain web application. This process ensures that all Thawing Systems in an organisation are running only those thawing protocols which have been approved by an appropriately qualified individual in that organisation. For details of how to get access to my.Cryochain, please see the following hyperlink

http://my.cryochain.com/

If a Thawing System is being used without the my.Cryochain software then protocols can be edited from the Admin menu. This is accessed by pressing and holding the circle button. The full procedure is shown here.